Captain Spaulding (Sid Haig), Otis Driftwood (Bill Moseley) and Baby (Sheri Moon Zombie) are on the run after the police raid the farmhouse. This is the story of their escape and the descent into madness of Sherriff Wydell (William Forsythe), brother of Lieutenant Wydell from House of 100 Corpses, slides into while hunting the family of murderers that killed his brother.

Two couples are traveling across the country and see a roadside attraction called “Captain Spaulding’s Gasoline and Chicken” Inside is a museum dedicated to every freaky thing you can think of. Captain Spaulding (Sid Haig) tells them about local legend Dr. Satan. The kids go to see the tree where Dr. Satan was hung and on the way they have a flat tire and are taken hostage by a family of sadistic freaks.